Sample must be cleaned and free of interferents. Polymerase Chain Reaction Fluorescence īIORAD - QX200 Auto Droplet Generator.pdf|BIORAD - QX200 Droplet Reader.pdf|ĭroplet Generator: 11 x 14 x 5 in (28 x 36 x 13 cm), Droplet reader: 26 x 20 x 11 in (66 x 52 x 29 cm) The fraction of PCR-positive droplets enables the target to be quantified according to the Poisson distribution. Positive droplets, which contain at least one copy of the target DNR or RNA molecule, exhibit increased fluorescence compared to negative droplets. The droplets stream single file through the reader for fluorescence analysis. Droplets are transferred to a 96-well PCR plate and PCR is performed to end point in a thermal cycler. The same system, which includes the QXDx Automated Droplet Generator and QXDx Droplet Reader, provides precise and absolute quantification of target DNA and RNA molecules in a wide variety of. ![]() ![]() Each droplet serves to partition the reactions. The QXDx AutoDG ddPCR System is Bio-Rad's Droplet Digital PCR system that is FDA-cleared for use with QXDx BCR-ABL IS Kit monitoring in the clinical lab. The QX200 droplet generator, which can process eight samples at a time and requires samples to be manually pipetted into sample wells, partitions each sample into 20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. The system consists of four instruments: a droplet generator, a plate sealer, a thermo cycler, and a droplet reader. Automated Droplet Generator uses microfluidics to combine oil and an aqueous sample to generate the nanoliter-sized droplets required for ddPCR. The Bio-Rad QX200 ddPCR System combines water-oil emulsion droplet technology with microfluidics. With the introduction of the QX600 Droplet Digital PCR System, BioRad continues to push the boundaries of digital PCR innovation with advanced, 6-color multiplexing.
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